Abstract: Biodegradation of Bitumen by aerophilic organisms was carried out by inoculating the bitumen sample on nutrient agar plate. The sample was divided into six as five parts were cultured aerobically while a part was cultured anaerobically using anaerobic jar. The isolation, characterization and identification of the microbial isolate revealed that five bacterial general: such as Pseudomonas aeroginosa, Bacillus subtilis, Bacillus cereus, Aerobacter aerogenes and Aerobacter cecacac are grown in each plate after 24 hours and recorded as positive (+). Conversely, characteristic of isolate was also investigated such as colour, shape, cell shape, spore shape and gram reaction, biochemical test was also carried out on each bacterial isolate such as motility test, coagulase test, indole and catalase test. However, result shows that sample A has (brown), B (white), C (white to yellow), D (thick white) and E (white spreading) colour, other morphological characteristics like shape and gram staining reaction are indicated as positive(+) and negative (-) respectively.