Abstract: Aim: This study aims to examine the effect of radial maze size (number of locations) on learning and performance efficiency of rats, i.e., do rats economize their physical effort when exploring the bigger maze and to assess the effect of sequence of training , 8-arm after 24-arm maze versus 24-arm after 8-arm maze, on relative performance of each rat in the two mazes. Materials and method: 22 Albino rats of Wistar strain obtained from National Institute of Nutrition Hyderabad, India were divided into two groups and were trained in 8-arm maze following training in 24-arm and vice-versa. The performance of the two groups were compared after obtaining plateau phase from the 10 trial moving average of Reference memory error for locating the onset of plateau phase. Results : One group of rat which were trained in 24 arm maze followed by 8-arm maze shows decrease in percentage Reference memory error in 8-arm maze .i.e., 2nd maze(P-value=0.003). The other group which were trained in 8-arm maze followed by training in 24-arm maze shows decrease in percentage Reference memory error in 24-arm i.e.2nd maze (P value= 0.001). Conclusion: The present study shows that rats after being trained in one radial maze perform better in another radial maze as far as Reference memory error in fully trained stage is concerned. This shows that repeated training with allothetic cues improves the ability to perform a task irrespective of maze size .