Abstract: The present study was to evaluate the wound healing activity of developed polyherbal formulation in the form of ointment by using hydroalcoholic extracts of leaves of Ficus religiosa, Mentha arvensis and roots of Rauwolfia serpentina with excipients. The phytochemical studies revealed the presence of alkaloids, phytosterol, tannins, flavonoids and protein. The developed polyherbal ointment was examined for wound healing activity in two types of wound models on rats; the excision and incision wound model. The polyherbal formulation was applied once daily until complete healing of wound and it was noted that the period of epithelization significantly (P < 0.05) reduced when compared with vehicle control group (15 ±0.34) which was nearly comparable with standard drug group. The tensile strength of healed wound was significantly increased (P < 0.05) as compared to vehicle control group. Hence the developed polyherbal formulation showed significant (P < 0.05) wound healing activity as compared to control group. The obtained results were nearly compared with standard drug group betadine ointment in terms of contractibility, wound closure time and tensile strength.