Abstract: Palm oil mill effluent (POME) is produced in large volumes by many of the palm oil mills in Malaysia and contributes a major source of pollution. The main aim of the present study was to evaluate the biodegradation potential of bacterial isolated from POME and to find the most suitable strain(s) for a biological treatment technology of POME. The isolates were identified by sequences analysis of 16S rRNA genes. Sequencing of the 16S rRNA of the isolates suggests that they were identified as Micrococcus luteus101PB, Stenotrophomonas maltophilia102PB, Bacillus cereus103PB, Providencia vermicola104PB, Klebsiella pneumoniae105PB and Bacillus subtilis106PB.Results revealed that total suspended solids (TSS), oil and grease were reduced dynamically with treatments after 5 days.