Abstract: The effects of different concentrations of copper sulphate on the growth of and the accumulation of Cu+2 by root, shoot, chlorophyll content and leaf growth of sunflower (Helianthus annus L.) were examined in this study. The purpose of our experiments was to consider sensitivity of two sunflower hybrid cultivars (FH626 and FH674) to copper ions on the ground of physiological characteristics amount of assimilatory pigment, and shooth length to show possible resistance mechanisms of this plant to copper ions. The experiment was conducted in Completely Randomized Fashion with three replications per treatment and five plants per replication.