Abstract: Cryptosporidium is a genus of protozoa of the Apicomplexa phylum with worldwide distribution that parasites the edges of intestinal microvilli causing a clinical presentation characterized by abdominal pains, vomiting and diarrhea, mainly affecting children and immunodeficient people. The objective of this research is to investigate oocysts of Cryptosporidium spp. in the feces of the Toba Qom indigenous people of San Francisco de Asis community, in the rural area of Benjamín Aceval city, Paraguay. 90 fecal samples were examined from people of both genders from 1 to 70 years old. The samples were processed by the Ritcher technique. Slides of the obtained sediment were stained by the Kynioun method and examined under light microscopy at 1000x magnification. Cryptosporidium spp. oocysts were found in 8 of the 90 fecal examined samples, which correspond to a prevalence coefficient (PC) of 8.9%. The research revealed the need to improve health care programs in order to provide better living conditions to people of San Francisco de Asis community.