Abstract: Bioacoustics is the science that combines biology and acoustics and refers to the production of sound, dispersion and reception by/to animals and humans. This study used acoustic and behavioral observations of dolphins to see the different patterns of sound, and describe behavior physiotherapy pool at Safari Park Indonesia, Cisarua Bogor. Power Spectral Density (PSD), the frequency range of the whistle sound different from one another. The highest intensity values that are at the sound of the whistle 3 after eat show pool with an intensity value of 25.57 dB at a frequency of 14470 Hz. Test result F before eating physiotherapy pool have in common (Homogeneous) contained in the relationship between the time 500 to 600 with the results of the whistle 1, 2, 3, and 4 are Reject Ho (Fhit> F table). Position of dolphins in a pool physiotherapy more dominant and often located in the bottom of the pool.