Abstract: The present experimental investigation has been carried out in order to explore the possible molecular interionic interactions of l-histidine in aqueous fructose solution at 298.15 K, 303.15K, 308.15K, and 313.15 K. Experimental values of density (ρ), ultrasonic speed (u) , and viscosity (η) have been measured on the liquid ternary mixtures of water +fructose + l-histidine and relevant molecular interaction parameters such as the apparent molal volume, Vφ , partial apparent molal volume, Vφ0 and the slope, Sv, Hepler’s constant, ∂2Vφ0/∂T2, apparent molal compressibiliity, Kφ , partial apparent molal compressibility, Kφ0 and the slope, Sk, transfer volume, Vφ0tr , transfer compressibility, Kφ0tr , Jones–Dole coefficient, B, Jones–Dole coefficient transfer, Btr , temperature derivative of B-coefficient, dB/dT, free energy of activation of viscous flow per mole of solvent, Δµ10* and per mole of solute, Δµ20* have been calculated. The results are interpreted in terms of solute–solvent and solute–solute interactions in these systems. It is observed that there exist strong solute–solvent interactions in these systems, which increase with increase in fructose concentration. The thermodynamics of viscous flow has also been discussed.