Abstract: Eichhornia crassipes (Mart.) Solms is a free-floating aquatic macrophyte growing normally to a height of 0.5 m to 1 m. This weed causes, substantial economic and ecological harm, warranting its removal, lest the impacts of the weed should become permanent. From an ecological perspective, the most effective, management approach is to make use of the weed for various purposes. The present study offers the novel scheme for utilization of roots of this invasive weed plant. In this instance the 80% methanol extract and methanol fraction from the roots of this weed were tested for its colouring properties on different textile substrates viz. wool, nylon, silk, cotton and polyester. Open beaker and infra red colouring based methods were utilized for colouring of different textile fabric substrates. The colour fastness testing and colorimetric evaluation of the coloured samples has been carried out employing standard methods. The 80% methanol extract as well as the methanol fraction exhibited good colouring property on wool, nylon and silk. The presence of alkaloids, flavonoids, tannins, terpenoids, quinones, phenols and other phytochemicals enables good colour yield/depth of 80% methanol extract and methanol fraction on substrates. Thus, the colour extract from the roots may prove to be effective for conventional colouring and may provide commercial importance of this natural colourant for textile applications.