Abstract: The development of differential pulse Polarographic (DPP) method for determination of riboflavin in pharmaceutical formulation was investigated. As first, we studied the electrochemical behaviour of riboflavin by DPP using a dropping mercury electrode (DME) as working and Hg/Hg2Cl2, Cl-(sat) as a reference electrode in Britton-Robinson (BR) buffer (pH 3.0−10). The results that were obtained showed that, the BR buffer solution with pH 7.0 was the best medium for reduction of riboflavin on the mercury electrode at peak potential (Ep) -1.48 V. The range of linearity was found to be from 1.0 to 16.0 ppm with limit of detection of (LOD) 0.915 ppm and limit of quantification (LOQ) was 3.050 ppm with R2 value was 0.997. Statistical analysis proved that the method was precise, reproducible, selective, specific, and accurate for analysis of riboflavin in pharmaceutical formulation.