Abstract: Antibiotics actively generated as staling growth product by the earlier established fungal colonies that inhibit the growth of soil inhabiting microfungi. In the present study, the composite soil mycoflora was assessed for its capability to get established on virgin and staled agar discs after 24, 48, 72, 96 and 120hrs under in vitro condition. It was experimentally monitored that the intricate pattern of fungal growth varied widely in each staled agar disc plate. The interim result from the experiment showed that the sum of fungi colonizing the staled agar disc reduces gradually with time from 24 to 120 hrs. Based on their racial low to high endurance capacity against the staled growth products or antibiotics actively generated by pre-colonized fungal colonies, they were sorted in VI groups.