Abstract: Needle prick injury poses a significant healthcare hazard amongst the health care workers. There is an increased risk of occupational transmission of blood borne pathogens subsequent to accidental needle prick injuries. Various studies indicate that the incidence of needle prick injury is on rise in the community of healthcare providers. The study was conducted for 120 hours in the department of anaesthesia, general surgery, gynaecology, orthopaedics and trauma centre of a tertiary care hospital to determine the incidence of needle prick injury, awareness amongst various health care workers about transmission of hepatitis B & HIV the two most dreaded diseases subsequent to needle prick injuries and awareness for post exposure prophylaxis and availing the same.