Abstract: Chalcone moiety (1,3‐diaryl‐2‐propen‐1‐ones) is the backbone of several antinuclear, cardiovascular and antispasmodic drugs. These groups of compounds constitute an important class of natural products belonging to the flavonoid family. A novel 1-(4-butoxy-2-hydroxy phenyl)-3-(2,5-dimethoxyphenyl) prop-2-en-1-one has been synthesized by chemical method. The product has been confirmed by their chemical analysis IR, 1H NMR and 13C NMR. To explore the structural features of the compound X-Ray diffraction (XRD) pattern has been recorded and it confirms that the title compound, C21H24O5 belongs to monoclinic system with P 2/m space group and the crystallographic parameters are a =22.4642 (0.0756), b = 17.1823 (0.0222), c = 10.3753 (0.0324) Ǻ and β = 92.93 (1.410) °. The final product has been screened for their antimicrobial and fungal activities against different panel of organisms.