Abstract: Callus cultures were established from hypocotyl and cotyledon explants of 8-10 days old seedlings obtained from non-irradiated and irradiated seeds of Brassica carinata variety ‘Jayanti’ on MS medium supplemented with various combinations and concentrations of growth hormones. Shoot formation from the cotyledon explants was observed on MS medium supplemented with BAP (2.0 mg l-1) and NAA (0.1 mg l-1) obtained from non-irradiated seeds. Multiple shoots appeared at the base of cotyledon explants of non-irradiated seeds within 3 weeks. AgNO3 in callus induction medium resulted in greening of callus. However, no significant effect of adding organic additive in shooting medium was observed. The hypocotyl explants showed good callusing but, no shooting response was observed. The callusing potential increased with increase in radiation dose up to 80 kR and afterwards started decreasing with increase in dose. The percentage of shoot formation decreased gradually with the increase in radiation dose up to 100 kR (except 60 kR) and the shoots developed were small, less vigorous with retarded growth and yellowish in color. Total suppression of shoot formation was observed in cultures derived from seeds treated with 110 kR dose.