Abstract: The aim of this study was to carry out comprehensive investigations on both microbiological and physicochemical characteristics of wastewater generated from catfish ponds within Oyo town in Oyo State. Data on microbiological concentration revealed that the wastewater from the ponds are highly loaded in organic pollutants and in pathogens bacteria, predominantly; Bacillius Spp., Pseudomonas Spp., Aeromonas Spp., Proteus Spp, Staphycoccus Spp showing that the water is not save for irrigation and when applied, the crop planted under the study area could be detrimental to the health of people when such crop consumed. Data was also collected and analyzed for a number of standard physicochemical parameters including pH, hardness, total dissolved solids, conductivity, alkalinity, chloride, nitrates, ammonia, sulphates, phosphates metals and heavy metals namely: Ca, Na, K, Mg, Fe, Cu, Zn, Al, Cr, Pb, Ni, Mn and Cd using standard analytical methods. And comparison was made between the parameters analyzed with World Health Organization recommended level and Nigerian Water Quality Standard. It was then revealed that majority of the physicochemical viz; magnesium, sodium, total dissolved solid were found to be within the Nigerian water standard limit, except in some view months where the average values were found to be higher than the recommended values.