Abstract: This study aims to determine the effect of defoliation intervals on the growth and quality of Brachiaria brizantha, Brachiaria decumbens, and Brachiaria decumbens cv.Mulato planted into polybags containing soil originating from critical dry land. This research was conducted by factorial completely randomized design (3x3) with 3 replications for each treatment combination. Factor A was defoliation interval i.e 30-, 45-, and 90-day age and Factor B was a grass species. The total unit of the experiment is 27. The results showed that the defoliation interval in grass species had significantly affected (P <0.01) plant height, number of tillers, leaf area, specific leaf area, dry weight, cumulative production, leaf chlorophyll content and crude protein content and so do both factors. Based on this study, defoliation interval of 90-day age has better response to plant growth and defoliation interval of 45-day age has better response to nutritional quality. Brachiaria brizantha grass has better growth and nutritional quality compared to other species grass.