Abstract: Investigated were four different samples of diseased cows, by means of fluorescence spectroscopy. It was found that the most suitable pumping wavelength fluorescence method to test samples of cows is 245 nm. Selected as low pumping wavelength, because the bacteria that cause diseases and infections in cattle have lower emission wavelengths. Through the application of fluorescence spectroscopy establishes accessible and rapid method for analysis of different samples of one animal unit. Equally effective were tested as a blood sample, and samples of abscess, vaginal and nasal discharge. Fluorescence spectroscopy was carried out at the dairy farm are not carried in the laboratory, it was possible because of the mobility of the test this device. The method can be applied to a mass testing because the pattern of spectral analyzer for fluorescence spectroscopy described in this article is easy to operate and quite affordable. Fluorescence spectroscopy can safely replace labor-intensive and expensive tests of trivial microbiology laboratories. The three main advantages of fluorescence spectroscopy is that the method is fast, does not require consumables and can be carried out at the dairy farm.