Abstract: The Aim of this conclusive research study was to examine factors affecting job satisfaction of Mekelle University academic staff as explained by Herzberg job motivator and hygiene factors. A random sample of 60university employees of Mekelle University was selected as a statistical sample. Employing a descriptive survey method and data were collected through questionnaire. The campus members were generally satisfied with their jobs. However, female faculty members were less satisfied than male faculty members. The factor “Achievement” was the most motivating aspect for faculty. The least motivating aspect was “salary.” The demographic characteristics were negligibly related to overall job satisfaction.